项目组成员结构完整、专业配备齐全、成熟，目前各级各类科研人员共计10余人。课题组科研人员熟练使用各种仪器和方法对小鼠以及人的干细胞细胞分离、培养以及移植，运用各种生化技术在干细胞水平上探究生命本质，并且可以使用影像技术手段能够直观展示生物代谢过程；采用生信大数据技术多方位多角度对各种生命现象进行解读。本课题组单细胞移植以及生物信息分析水平居于世界领先水平。

在研项目：

1.单细胞水平解析小鼠造血干细胞移植后重建动力学规律（国自然面上项目）

2 运用CRISPR-Cas9基因编辑技术研究多基因对造血干细胞协（国自然面上项目）

3 单细胞水平MDS高频突变基因的造血谱系检测及临床相关性研究（国自然面上项目）

4 血细胞的体外规模化制备及功能研究（国防科技创新特区163计划）

5 建立与优化HSC和ES来源的NSC的扩增及质控体系（国家重点研发计划））

6 功能性免疫细胞再生技术（国家重点研发计划）

7 干细胞损伤、修复及组织重建的基础与转化研究（创新工程）

8 血细胞图谱项目（创新工程）

研究经费充足，目前总计在研经费1200多万元。

01. 2020-2023   国自然面上项目：单细胞水平MDS高频突变基因的造血谱系检测及临床相关性研究     55万   负责人

02. 2017-2021   国家重点研发计划：建立与优化HSC和ES来源的NSC的扩增及质控体系                    300万   负责人

03. 2017-2022   创新工程：血细胞图谱项目                                                                                          220万    负责人

04. 2017-2020   国自然面上项目：运用CRISPR-Cas9基因编辑技术研究多基因对造血干细胞协           55万   负责人

05. 2019-2020   医科院基本科研业务费：造血干细胞体外扩增及临床应用的研究                                   60万    负责人

06. 2015-2018   国家自然科学基金面上项目：IL-12 高效扩增造血干细胞及机制研究                              90万    负责人

07. 2015-2019   国家重点研发计划：造血干细胞移植的免疫学基础研究                                                131万    负责人

08. 2014-2018   国家重点实验室自主课题：造血干细胞体外扩增及机制研究                                         200万    负责人

09. 2013-2017   基础研究：单个造血细胞的自我更新诱导                                                             1400万日元    负责人

10. 2011-2012 新学术领域研究：造血干细胞分化的多样性                                                            200万日元    负责人  

11. 2010-2011 特定领域研究：G2信号在决定造血干细胞命运中的探索                                         600万日元    负责人

12.2010-2012    基础研究：造血干细胞的DNA甲基化                                                                    400万日元    负责人 

13. 2008-2009   特定研究：两个不同功能的龛                                                                                740万日元   负责人        

14. 2006-2007   特定研究：调控造血干细胞不对称分裂的细胞外因子                                            780万日元   负责人

15. 2004-2005   基础研究：Wnt在造血干细胞调控中的作用                                                           360万日元    负责人                                          

1: Xie M, Zhang S, Dong F, Zhang Q, Wang J,Wang C, Zhu C, Zhang S, Luo B, Wu P, Ema H. G-CSF directly acts on

 mouse lymphoid-biased but not myeloid-biasedhematopoietic stem cells. Haematologica. 2020 Feb PMID: 32079694.

2: Wang J, Liu Z, Zhang S, Wang X, Bai H,Xie M, Dong F, Ema H. Lineage marker expression on mouse hematopoietic

 stemcells. Exp Hematol. 2019 Aug;76:13-23.e2.doi: 10.1016/j.exphem.2019.07.001.Epub 2019 Jul 9. PMID: 31299288.

3: Dong F, Bai H, Wang X, Zhang S, Wang Z,Xie M, Zhang S, Wang J, Hao S, Cheng T, Ema H. Mouse acute leukemia

develops independent of self-renewal anddifferentiation potentials in hematopoietic stem and progenitor cells.

 Blood Adv. 2019 Feb 12;3(3):419-431. doi:10.1182/bloodadvances.2018022400. PMID:30733302; PMCID: PMC6373745.

4: Wang X, Dong F, Zhang S, Yang W, Yu W,Wang Z, Zhang S, Wang J, Ma S, Wu P, Gao Y, Dong J, Tang F, Cheng T, Ema

 H.TGF-β1 Negatively Regulates the Number andFunction of Hematopoietic Stem Cells. Stem Cell Reports. 2018 Jul 10;

11(1):274-287. doi:10.1016/j.stemcr.2018.05.017. Epub 2018 Jun 21. PMID: 29937145; PMCID:PMC6067088.

5: Wang X, Gong Y, Ema H. Chasing theprecursor of functional hematopoietic stem cells at the single cell levels inmouse

 embryos. J Hematol Oncol. 2016 Jul 22;9(1):58.doi: 10.1186/s13045-016-0289-7. PMID: 27448408; PMCID: PMC4957898.

6: Ema H. Guest editorial: Institute ofHematology and Blood Diseases Hospital in China. Int J Hematol. 2016May;103(5):

486. doi: 10.1007/s12185-016-1967-5.Epub2016 Mar 10. PMID: 26964778.

7: Ema H, Uchinomiya K, Morita Y, Suda T,Iwasa Y. Repopulation dynamics of single haematopoietic stem cells in mouse

 transplantation experiments:Importance of stemcell composition in competitor cells. J Theor Biol. 2016 Apr 7;394:57-67.

 doi:10.1016/j.jtbi.2016.01.010. Epub 2016 Jan 20. PMID: 26802482.

8: Wang Z, Ema H. Mechanisms ofself-renewal in hematopoietic stem cells. Int J Hematol. 2016May;103(5):498-509. doi:

10.1007/s12185-015-1919-5. Epub 2015 Dec12. PMID: 26662558.

9: Ema H, Morita Y, Suda T. Heterogeneityand hierarchy of hematopoietic stem cells. Exp Hematol. 2014 Feb;42(2):74-82.

e2. doi: 10.1016/j.exphem.2013.11.004.Epub2013 Nov 20. PMID: 24269919.

10: Yamamoto R, Morita Y, Ooehara J,Hamanaka S, Onodera M, Rudolph KL, Ema H,Nakauchi H. Clonal analysis unveils

self-renewing lineage-restrictedprogenitors generated directly from hematopoietic stem cells. Cell. 2013 Aug 29;154(5):

1112-1126. doi: 10.1016/j.cell.2013.08.007.PMID: 23993099.

11: Ema H, Suda T. Two anatomicallydistinct niches regulate stem cell activity. Blood. 2012 Sep13;120(11):2174-81.

doi: 10.1182/blood-2012-04-424507. Epub2012 Jul 11. PMID: 22786878.

12: Yamazaki S, Ema H, Karlsson G,Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, Nakauchi H.

Nonmyelinating Schwann cells maintain hematopoieticstem cell hibernation in the bone marrow niche. Cell. 2011 Nov

15;147(5):1146-58. doi:10.1016/j.cell.2011.09.053. PMID: 22118468.

13: Morita Y, Iseki A, Okamura S, Suzuki S,Nakauchi H, Ema H. Functional characterization of hematopoietic stem cells

in the spleen. Exp Hematol. 2011 Mar;39(3):351-359.e3.doi: 10.1016/j.exphem.2010.12.008. Epub 2010 Dec 24. PMID:

21185906.

14: Morita Y, Ema H, Nakauchi H.Heterogeneity and hierarchy within the most primitive hematopoietic stem cell

compartment. J Exp Med. 2010 Jun 7;207(6):1173-82.doi: 10.1084/jem.20091318. Epub 2010 Apr 26. PMID: 20421392;

PMCID: PMC2882827.

15: Matsumoto K, Isagawa T, Nishimura T,Ogaeri T, Eto K, Miyazaki S, Miyazaki J, Aburatani H, Nakauchi H, Ema 

H.Stepwise development of hematopoietic stem cells fromembryonic stem cells. PLoS One. 2009;4(3):e4820. doi:

10.1371/journal.pone.0004820. Epub 2009 Mar 16. PMID:19287487; PMCID:PMC2653650.

16: Ema H, Nakauchi H. Bloodlines ofhaematopoietic stem cell research in Japan.Philos Trans R Soc Lond B Biol Sci.2008

 Jun27;363(1500):2089-97. doi:10.1098/rstb.2008.2263. PMID: 18375375; PMCID:PMC2610182.

17: Ema H, Morita Y, Yamazaki S, MatsubaraA, Seita J, Tadokoro Y, Kondo H,Takano H, Nakauchi H. Adult mouse 

hematopoietic stemcells: purification and single-cell assays. Nat Protoc. 2006;1(6):2979-87. doi:10.1038/nprot.

2006.447.PMID: 17406558.

18: Ema H, Morita Y, Nakauchi H, MatsuzakiY. Isolation of murine hematopoietic stem cells and progenitor cells. CurrProtoc

Immunol. 2005 Jul;Chapter 22:Unit 22B.1.doi: 10.1002/0471142735.im22b01s67. PMID: 18432947.

19: Ema H, Sudo K, Seita J, Matsubara A,Morita Y, Osawa M, Takatsu K, Takaki S, Nakauchi H. Quantification of 

self-renewal capacity in single hematopoietic stem cellsfrom normal and Lnk-deficient mice. Dev Cell. 2005 Jun;8(6):

907-14. doi:10.1016/j.devcel.2005.03.019. PMID:15935779.

20: Ema H, Kinda M, Takano Y, Oehara A,Maeda A, Nakauchi H. [Diversity of hematopoietic stem cells]. Rinsho 

Ketsueki.2005 Mar;46(3):170-8. Japanese.PMID: 16447711.

21: Ema H. [Mechanisms controlling the fateof hematopoietic stem cells]. Tanpakushitsu Kakusan Koso. 2004May;49(6):734-

40.Japanese. PMID: 15160882.

22: Ema H, Nakauchi H. "Homing toNiche," a new criterion for hematopoietic stem cells? Immunity. 2004Jan;20(1):1-2. doi:

10.1016/s1074-7613(04)00002-0.PMID:14738758.

23: Ema H, Nakauchi H. Self-renewal andlineage restriction of hematopoietic stem cells. Curr Opin Genet Dev. 2003 Oct;

13(5):508-12.doi:10.1016/j.gde.2003.08.011. PMID: 14550417.

24: Nakauchi H, Sudo K, Ema H. Quantitativeassessment of the stem cell self renewal capacity. Ann N Y Acad Sci. 2001 Jun;

938:18-24; discussion 24-5.doi:10.1111/j.1749-6632.2001.tb03570.x. PMID: 11458506.

25: Ema H, Takano H, Sudo K, Nakauchi H. Invitro self-renewal division of hematopoietic stem cells. J Exp Med. 2000 Nov 6;

192(9):1281-8. doi:10.1084/jem.192.9.1281.PMID: 11067877; PMCID: PMC2193353.

26: Ema H, Nakauchi H. Expansion ofhematopoietic stem cells in the developing liver of a mouse embryo. Blood. 2000Apr 1;

95(7):2284-8. PMID: 10733497.

27: Ema H, Douagi I, Cumano A, Kourilsky P.Development of T cell precursor activity in the murine fetal liver. Eur J Immunol.

 1998May;28(5):1563-9.doi:10.1002/(SICI)1521-4141(199805)28:05<1563::AID-IMMU1563>3.0.CO;2-R.PMID:9603461.

28: Ema H, Cumano A, Kourilsky P. TCR-betarepertoire development in the mouse embryo. J Immunol. 1997 Nov 1;159(9):

4227-32. PMID: 9379017.

29: Ema H, Suda T, Nakauchi H, Nakamura Y,Iwama A, Imagawa S, Akutsu M, Kano Y, Kato S, Yabe M, et al. Multipotent and

committed CD34+ cells in bone marrow transplantation.Jpn J Cancer Res. 1991 May;82(5):547-52. doi:10.1111/j.1349-7006.

1991.tb01885.x. PMID: 1712005; PMCID:PMC5918482.

30: Ema H, Suda T, Nagayoshi K, Miura Y,Civin CI, Nakauchi H. Target cells for granulocyte colony-stimulating factor,

interleukin-3, and interleukin-5 in differentiationpathways of neutrophils and eosinophils. Blood. 1990 Nov

15;76(10):1956-61. PMID: 1700728.

31: Ema H, Suda T, Miura Y, Nakauchi H.Colony formation of clone-sorted human hematopoietic progenitors. Blood. 1990

May 15;75(10):1941-6. PMID: 1692488.

32: Ema H, Kitano K, Suda T, Sato Y, MuroiK, Ohta M, Yoshida M, Sakamoto S, Eguchi M, Miura Y. In vitro differentiationof

 leukemic cells to eosinophils in the presenceof interleukin-5 in two cases of acute myeloid leukemia with the translocation

 (8;21)(q22;q22). Blood. 1990 Jan15;75(2):350-6. PMID: 1688504.

33: Ema H, Suda T, Sakamoto S, Tomonaga T,Tsunoda J, Muroi K, Komatsu N, Miwa A, Ohsaka A, Yoshida M, et al. Effects 

of the in vivo administration of recombinanthuman granulocyte colony-stimulating factor following cytotoxic chemotherapy on

granulocytic precursors in patients withmalignant lymphoma. Jpn J Cancer Res. 1989 Jun;80(6):577-82. doi:10.1111/j.1349-

7006.1989.tb01678.x.PMID: 2474526;