作为负责人，主持课题研究10多项，主要包括北京市自然科学基金、协和青年科研基金、美国中华医学基金会（CMB）基金项目等。作为主要参加人，参加课题研究20多项，主要包括“重大新药创制”国家科技重大专项基金项目、国家自然科学基金、中国医学科学院医学与健康科技创新工程重大协同创新项目等。

近年承担主要科研项目情况（项目名称/项目来源/本人角色/起止时间）：

1. 新型聚乙烯亚胺衍生物非病毒载体的合成及其递送siRNA药物的抗肿瘤研究，北京市自然科学基金面上项目，项目主持人，2014.01至2016.12

2. 丝裂霉素C脑靶向纳米释药系统的构建及治疗胶质母细胞瘤的研究，北京协和医学院协和青年科研基金，项目主持人，2017.04至2018.12

3. 非病毒载体(PEG5K-PCL1.2K)1.4-g-PEI10K的转染机制及递送功能siRNA治疗恶性肿瘤的研究，北京协和医学院协和青年科研基金，项目主持人，2012.10至2013.09

4. siRNA药物新型高效纳米载体的研究，中央级公益性科研院所基本科研业务费专项科研重点项目，项目主持人，2014.01至2015.12

5. 罕见病及协和特色制剂等特殊品种和关键技术研究，中国医学科学院医学与健康科技创新工程重大协同创新项目，主要参与（任务四负责人），2017.01至2020.12

6. 维药香青兰有效部位复合磷脂脂质体纳米制剂的研发，新疆维吾尔自治区科技支疆项目计划（指令性）项目，主要参与（副组长），2015.01至2017.12

7. 香青兰有效部位滴丸临床前研究，新疆维吾尔自治区科技计划高技术研究发展项目，主要参与（副组长），2015.01至2017.12

8. 抗老年痴呆及轻度认知障碍新药sGC-1061的临床前研究，十二五“重大新药创制”国家科技重大专项子课题，主要参与，2014.01至2016.12

9. 高良姜素诱导肝癌细胞自噬死亡的途径及相关分子机制的研究，国家自然科学基金面上项目，主要参与，2013.01至2016.12

10. 以胆盐为靶头的新型天然生物大分子口服纳米递送系统的构建和体内外研究，天然药物活性物质与功能国家重点实验室自主课题重点项目，主要参与，2018.01至2020.12

11. 肿瘤免疫治疗疫苗PIC纳米制剂的制备及其抗乳腺癌生长和转移的研究，天然药物活性物质与功能国家重点实验室自主课题C类探索性研究课题，主要参与，2017.01至2021.12

12. 帕米卡抗肿瘤药效学评价，技术服务合同项目（委托单位：信福（北京）医药科技有限公司），主要参与，2018.03至2019.02

13. 药物制剂及新型释药系统，协和学者与创新团队发展计划，主要参与，2013.10至2016.09

14. 药用聚合物mPEG-b-PDLLA及其载抗肿瘤药物多西他赛胶束纳米制剂的临床前研究，十二五“重大新药创制”国家科技重大专项创新药物研究开发技术平台建设创新药物研发课题，主要参与，2013.01至2015.12

15. 基于适体构建抗前列腺癌纳米靶向胶束，天然药物活性物质与功能国家重点实验室自主课题重点项目，主要参与，2016.01至2017.12

16. LX2-32C聚乙二醇化白蛋白纳米粒的研究，天然药物活性物质与功能国家重点实验室自主课题C类探索性研究课题，主要参与，2012.01至2016.12

在国内进行了基因递送载体的开拓性研究，率先研制壳聚糖纳米粒基因递送系统。在基因递送载体、纳米释药系统和创新制剂等方面取得重要研究成果，申请发明专利20多项，发表论文50多篇，多篇SCI论文发表于Biomaterials、 Advanced Drug Delivery Reviews、International Journal of Pharmaceutics、Theranostics、International Journal of Nanomedicine、Acta Biomaterialia、European Journal of Pharmaceutical Sciences等。

近年代表性论文 (#co-first author, *corresponding author)：

1)    Wei Huang#, Liqing Chen#, Lin Kang#, Mingji Jin, Ping Sun, Xin Xin, Zhonggao Gao*, You Han Bae. Nanomedicine-based combination anticancer therapy between nucleic acids and small-molecular drugs. Advanced Drug Delivery Reviews, 2017, 115:82-97. (IF: 13.66)

2)    Bo Fan, Lin Kang, Liqing Chen, Ping Sun, Mingji Jin, Qiming Wang, You Han Bae, Wei Huang*, Zhonggao Gao*. Systemic siRNA delivery with a dual pH-responsive and tumor-targeted nanovector for inhibiting tumor growth and spontaneous metastasis in orthotopic murine model of breast carcinoma. Theranostics, 2017, 7(2):357-376. (IF: 8.537)

3)    Feifei Yang#, Wei Huang# , Yunfei Li, Shan Liu, Mingji Jin, Yuli Wang, Lihua Jia, Zhonggao Gao*. Anti-tumor effects in mice induced by survivin-targeted siRNA delivered through polysaccharide nanoparticles. Biomaterials, 2013, 34(22):5689-5699. (IF: 8.312)

4)    Xiao-yang Chu#, Wei Huang#, Yu-li Wang, Ling-wei Meng, Li-qing Chen, Ming-ji Jin, Lu Chen, Chun-hong Gao, Cheng Ge, Zhong-gao Gao*, Chun-sheng Gao*. Improving anti-tumor outcomes for palliative intratumoral injection therapy through lecithin-chitosan nanoparticles loading paclitaxel-cholesterol complex. International Journal of Nanomedicine, 2019, 14:689-705. (IF: 4.471)

5)    Lingwei Meng, Xiaoyang Chu, Haoyue Xing, Xuan Liu, Xin Xin, Liqing Chen, Mingji Jin,Youyan Guan*, Wei Huang*, Zhonggao Gao*. Improving glioblastoma therapeutic outcomes via doxorubicin-loaded nanomicelles modified with borneol. 2019, International Journal of Pharmaceutics, 567: 118485. (IF: 4.213)

6)    Mingji Jin, Guangming Jin, Lin Kang, Liqing Chen, Zhonggao Gao*, Wei Huang*. Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes. International Journal of Nanomedicine, 2018, 13:2405-2426. (IF: 4.471)

7)    Ping Sun, Wei Huang*, Lin Kang, Mingji Jin, Bo Fan, Hongyan Jin, Qiming Wang, Zhonggao gao*. siRNA-loaded poly(histidine-arginine)6-modifed chitosan nanoparticle with enhanced cell-penetrating and endosomal escape capacities for suppressing breast tumor metastasis. International Journal of Nanomedicine, 2017, 12:3221-3234. (IF: 4.37)

8)    Liqing Chen, Wei Huang*, Zhonggao Gao*, Weishuo Fang, Mingji Jin. Lx2-32c-loaded polymeric micelles with small size for intravenous drug delivery and their inhibitory effect on tumor growth and metastasis in clinically associated 4T1 murine breast cancer. International Journal of Nanomedicine, 2016, 11:5457-5472. (IF: 4.3)

9)    Shan Liu#, Wei Huang#, Mingji Jin, Bo Fan, Guimin Xia, Zhonggao Gao*. Inhibition of murine breast cancer growth and metastasis by survivin-targeted siRNA using disulfide cross-linked linear PEI. European Journal of Pharmaceutical Sciences, 2016, 82:171-182. (IF: 3.756)

10) Cheng Zeng, Wen Jiang, Meie Tan, Xiaoyi Yang, Chenghui He, Wei Huang*, Jianguo Xing*. Optimization of the process variables of tilianin-loaded composite phospholipid liposomes based on response surface-central composite design and pharmacokinetic study. European Journal of Pharmaceutical Sciences, 2016, 85:123-131. (IF: 3.756)

11) Li-qing Chen#, Zhe-ming Zhang#, Wei Huang*, Zhong-gao Gao*, Wei-shuo Fang, Ming-ji Jin, Lian Yu. Inhibiting the proliferation, migration and invasion of triple negative breast cancer cells through anti-tumor human serum albumin nanoparticles loading aziditaxel as a novel taxane derivative. Pharmazie, 2017, 72(3):152-160. (IF: 1.016)

12) Mei-e Tan, Cheng-hui He, Wen Jiang, Cheng Zeng, Ning Yu, Wei Huang*, Zhong-gao Gao, Jian-guo Xing*. Development of solid lipid nanoparticles containing total flavonoid extract from Dracocephalum moldavica L. and their therapeutic effect against myocardial ischemia-reperfusion injury in rats. International Journal of Nanomedicine, 2017, 12:3253-3265. (IF: 4.37)

13) Yuli Wang#, Ning Yu#, Rui Guo#, Meiyan Yang, Li Shan, Wei Huang*,Wei Gong, Shuai Shao, Xiaoping Chen, Chunsheng Gao*. Enhancing in vivo bioavailability in beagle bogs of GLM-7 as a novel anti-leukemia drug through a self-emulsifying drug delivery system for oral delivery. Current Drug Delivery, 2016, 13:131-142. (IF: 2.516)

14) Yuli Wang, Jiahui Yang, Yong Qian, Meiyan Yang, Yan Qiu, Wei Huang*, Li Shan, Chunsheng Gao*. Novel ethylcellulose-coated pellets for controlled release of metoprolol succinate without lag phase: characterization, optimization and in vivo evaluation. Drug Development and Industrial Pharmacy, 2015, 41(7):1120-1129. (IF: 2.429)

15) Ning Yu, ChenHui He, Gulistan Awuti, Cheng Zeng, JianGuo Xing*, Wei Huang*. Simultaneous Determination of Six Active Compounds in Yixin Badiranjibuya Granules, a Traditional Chinese Medicine, by RP-HPLC-UV Method. Journal of Analytical Methods in Chemistry, 2015:974039. (IF: 1.369)

16) Yedan Wu, Wei Huang, Liqing Chen, Mingji Jin, Zhonggao Gao*, Changshan An*, Haixiang Lin. Anti-tumor outcome evaluation against non-small cell lung cancer in vitro and in vivo using PolyI:C as nucleic acid therapeutic agent. American Journal of Translational Research, 2019, 11(4): 1919-1937. (IF: 3.061)

17) Mengjiao Wang, Liqing Chen, Wei Huang, Mingji Jin, Qiming Wang, Zhonggao Gao*, Zhehu Jin*. Improving the anti-keloid outcomes through liposomes loading paclitaxel–cholesterol complexes. International Journal of Nanomedicine, 2019,14:1385-1400. (IF: 4.37)

18) Wenyu Jin, Wei Huang, Liqing Chen, Mingji Jin, Qiming Wang, Zhonggao Gao*, Zhehu Jin*. Topical application of JAK1/JAK2 inhibitor momelotinib exhibits significant anti-Inflammatory responses in DNCB-induced atopic dermatitis model mice. International Journal of Molecular Sciences, 2018, 19(12), 3973. (IF: 3.687)

19) Qiming Wang, Zhonggao Gao*, Shan Liu, Bo Fan, Lin Kang, Wei Huang, Mingji Jin. Hybrid polymeric micelles based on bioactive polypeptides as pH-responsive delivery systems against melanoma. Biomaterials, 2014, 35(25):7008-7021. (IF: 8.557)

20) Lin Kang, Bo Fan, Ping Sun, Wei Huang, Mingji Jin, Qiming Wang, Zhonggao Gao*. An effective tumor-targeting strategy utilizing hypoxia-sensitive siRNA delivery system for improved anti-tumor outcome. Acta Biomaterialia, 2016, 44:341-354. (IF: 6.319)

21) Holly Y. Chen#, Suk Ling Ma#, Wei Huang, Lindan Ji, Vincent H. K. Leung, Honglin Jiang, Xiaoqiang Yao, Nelson L. S. Tang*. The mechanism of transactivation regulation due to polymorphic short tandem repeats (STRs) using IGF1 promoter as a model. Scientific Reports, 2016, 6:38225. (IF: 4.259)

22) Ping Sun, Wei Huang, Mingji Jin, Qiming Wang, Bo Fan, Lin Kang, Zhonggao Gao*. Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis. International Journal of Nanomedicine, 2016,11:4931-4945. (IF: 4.3)

23) Mingji Jin, Guangming Jin, Wei Huang, Zhonggao gao*. PEGylation of Lumbrokinase improves pharmacokinetic profile and enhances anti-thrombotic effect in a rat carotid artery thrombosis model. Molecular Medicine Reports, 2017, 16(4):4909-4914. (IF: 1.922)

24) Shan Liu, Wei Huang, Mingji Jin, Qiming Wang, Ganlin Zhang, Xiaomin Wang, Shuai Shao, Zhonggao Gao*. High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect. International Journal of Nanomedicine, 2014, 9:3567-3581. (IF: 4.383)

25) Yunfei Li, Mingji Jin, Shuai Shao, Wei Huang, Feifei Yang, Wei Chen, Shenghua Zhang, Guimin Xia, Zhonggao Gao*. Small-sized polymeric micelles incorporating docetaxel suppress distant metastases in the clinically-relevant 4T1 mouse breast cancer model. BMC Cancer, 2014, 14:329. (IF: 3.362)

26) Holly Y. Chen, Wei Huang, Vincent H. K. Leung, Simon L. M. Fung, Suk Ling Ma, Hongling Jiang, Nelson L. S. Tang*. Functional interaction between SNPs and microsatellite in the transcriptional regulation of insulin-like growth factor 1. Human Mutation, 2013, 34(9):1289-1297. (IF: 5.05)

27) Yunfei Li, Feifei Yang, Wei Chen, Jiaoyang Liu, Wei Huang, Mingji Jin, and Zhonggao Gao*. A novel monomethoxy polyethylene glycol-polylactic acid polymeric micelles with higher loading capacity for docetaxel and well-reconstitution characteristics and its anti-metastasis study. Chemical & Pharmaceutical Bulletin, 2012, 60(9):1146-1154. (IF: 1.564)

28) Wei Huang, Ming Lv, Zhong-gao Gao, Ming-ji Jin, Yuan-ji Xu, Xiao-dan Yu, Zhe-hu Jin, Xue-zhe Yin. Preparation and characterization of polymeric nanoparticles for siRNA delivery to down-regulate the expressions of exogenous and endogenous target genes. Pharmazie, 2012, 67(8):676-680. (IF: 0.962)

29) Suk Ling Ma, Wei Huang, Nelson L.S. Tang*, Linda C.W. Lam. MxA polymorphisms are associated with risk and age-at-onset in Alzheimer disease and accelerated cognitive decline in Chinese elders. Rejuvenation Research, 2012, 15(5):516-522. (IF: 2.919)

30) Wei Huang, Ming Lv, Zhong Gao Gao*. Synthesis of polyethylenimine grafted with copolymers of polyethylene glycol and polycaprolactone and its potential for siRNA delivery. Chinese Chemical Letters, 2011, 22(6):749-752.

31) Lin Kang, Zhonggao Gao*, Wei Huang, Mingji Jin, Qiming Wang. Nanocarrier-mediated co-delivery of chemotherapeutic drugs and gene agents for cancer treatment. Acta Pharmaceutica Sinica B, 2015, 5(3):169-175.

32) Mingji Jin, Wei Chen, Wei Huang, Long Rong, Zhonggao gao*. Preparation of pegylated lumbrokinase and an evaluation of its thrombolytic activity both in vitro and in vivo. Acta Pharmaceutica Sinica B, 2013, 3(2):123-129.