1. 张沫, 副研究员，理学博士，硕士生导师

研究方向：肿瘤标志物的标记与检测的新技术新方法

2015年毕业于清华大学化学系，获得理学博士学位。同年进入中国医学科学院基础医学研究所生物物理学系工作。先后主持和作为主要成员参与了国家自然科学基金青年项目、面上项目，发表了30余篇SCI论文。

联系方式：

中国医学科学院基础医学研究所&北京协和医学院基础学院  生物物理学及结构生物学系  北京市东城区东单三条五号，100005 电话：010-69156458 E-mail：zhangmo234@126.com

代表性论文：

1.        Mo Zhang, Qing Wang, Yupin Xu, Lei Guo, Zhizhen Lai and Zhili Li*, Graphitic carbon nitride quantum dots as analytical probe for viewing sialic acid on the surface of cells and tissues, Analytica Chimica Acta, 2020, 1095,204-211.

2.        Mo Zhang, Yujie Liu, Dan Zhang, Tianjing Chen, Zhili Li*, Facile and Selective Enrichment of Intact Sialoglycopeptides Using Graphitic Carbon Nitride, Analytical Chemistry, 2017, 89(15) , 8064-8069.

3.        Zhang, Mo, Luo, Wenjiao, Wei, Zhen, Jiang, Wenjun, Liu, Di, Zhu, Yongfa*, Separation free C3N4/SiO2 hybrid hydrogels as high active photocatalysts for TOC removal, Applied Catalysis B-Environmental, 2016,194, 105-110

4.        Zhang, Mo, Jiang, Wenjun, Liu, Di, Wang, Jun, Liu, Yanfang, Zhu, Yanyan, Zhu, Yongfa*, Photodegradation of phenol via C3N4-agar hybrid hydrogel 3D photocatalysts with free separation，Applied Catalysis B-Environmental, 2016, 183, 263-268

5.        Zhang, Mo, Bai, Xiaojuan, Liu, Di, Wang, Jun, Zhu, Yongfa*, Enhanced catalytic activity of potassium-doped graphitic carbon nitride induced by lower valence position, Applied Catalysis B-Environmental, 2015, 164, 77-81

6.        Zhang, Mo，Yao, Wenqing，Lv, Yanhui，Bai, Xiaojuan，Liu, Yanfang，Jiang, Wenjun，Zhu, Yongfa*, Enhancement of mineralization ability of C3N4 via a lower valence position by a tetracyanoquinodimethane organic semiconductor，Journal of Materials Chemistry A, 2014, 2 (29), 11432-11438

7.        Zhang, Mo, Xu, Jing, Zong, Ruilong, Zhu, Yongfa*, Enhancement of visible light photocatalytic activities via porous structure of g-C3N4, Applied Catalysis B-Environmental, 2014, 147, 229-235

8.        Zhang, Mo, Bai, Linling, Shang, Weihu, Xie, Wenjing, Ma, Hong, Fu, Yingyi, Fang, Decai, Sun, Hui, Fan, Louzhen, Han, Mei，Liu, Chenmin, Yang, Shihe*, Facile synthesis of water-soluble, highly fluorescent graphene quantum dots as a robust biological label for stem cells, Journal of Materials Chemistry, 2012, 22 (15), 7461-7467

2. 周巾煜，博士, 助理研究员

2015年毕业于中国海洋大学药学专业,获得医学学士学位；2020年毕业于中国科学院生物物理研究所生物化学与分子生物学专业，获得理学博士学位，研究方向是糖组学与糖蛋白组学的质谱技术研究；同年8月入职中国医学科学院基础医学研究所生物物理学系。

已发表论文：

Zhou J, Gao H, Xie W, et al. Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants [J]. Applied Microbiology and Biotechnology, 2020, 104: 1583-1594.

Zhou J, Gao H, Xie W, et al. FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides[J]. Biochemical and Biophysical Research Communications, 2019, 520: 8-13.

周巾煜，李倩倩，黄纯翠等. 细菌脂多糖及其寡糖结构分析技术研究进展[J]. 生物化学与生物物理进展, 2017, 44: 49-57.

3. 李丹,  博士,  技术员

2011年毕业于鲁东大学化学专业,获得学士学位；2016年毕业于北京师范大学有机化学专业，获得理学博士学位；同年进入中国农业大学开展博士后工作；2019年10月入职中国医学科学院基础医学研究所生物物理学系。

1. 中国医学科学院医学与健康科技创新工程项目：血液中的疾病相关大规模糖组学数据人工智能识别技术研究与应用。

2. 中国医学科学院医学与健康科技创新工程项目： 肝癌新型分子标志物研发及风险预测模型。

3.  国家自然科学基金委员会：肺癌、胃癌、结直肠癌和多发性骨髓瘤预后评估个体化标志物及其快速检测方法研究。

4.  国家自然科学基金委员会：基于FTICR MS的慢性疾病特异性免疫球蛋白G的结构表征方法研究及其应用。

5.  国家自然科学基金委员会：应用质谱成像技术原位表征肺癌组织区域的巨噬细胞免疫特性。

6.  北京市自然科学基金委员会：肺癌个体化预后标志物的验证。

7.  国家自然科学基金委员会：应用质谱技术研究血清/血浆中与肿瘤相关的蛋白质复合物结构。

8.  国家自然科学基金委员会: 应用质谱成像技术探究非可控炎症恶性转化相关的物质基础。

9.  国家自然科学基金委员会：应用质谱技术探究胰腺癌相关蛋白酶体复合体。

10.  科技部，国家高科技研究发展计划(863)：肿瘤细胞泛素-蛋白酶体降解途径中相关新蛋白筛选技术研究。

11. 北京市自然科学基金委员会：胰腺癌生物标志物筛选。

12.   北京市自然科学基金委员会：几种常见肿瘤的分子标志谱建立及在预警、诊治和预后中的应用-胰腺癌分子标志谱的研究与分离鉴定肿瘤相关蛋白技术平台的建立。

1. 中国医学科学院医学与健康科技创新工程项目：血液中的疾病相关大规模糖组学数据人工智能识别技术研究与应用。

2. 中国医学科学院医学与健康科技创新工程项目： 肝癌新型分子标志物研发及风险预测模型。

3.  国家自然科学基金委员会：肺癌、胃癌、结直肠癌和多发性骨髓瘤预后评估个体化标志物及其快速检测方法研究。

4.  国家自然科学基金委员会：基于FTICR MS的慢性疾病特异性免疫球蛋白G的结构表征方法研究及其应用。

5.  国家自然科学基金委员会：应用质谱成像技术原位表征肺癌组织区域的巨噬细胞免疫特性。

6.  北京市自然科学基金委员会：肺癌个体化预后标志物的验证。

7.  国家自然科学基金委员会：应用质谱技术研究血清/血浆中与肿瘤相关的蛋白质复合物结构。

8.  国家自然科学基金委员会: 应用质谱成像技术探究非可控炎症恶性转化相关的物质基础。

9.  国家自然科学基金委员会：应用质谱技术探究胰腺癌相关蛋白酶体复合体。

10.  国家高科技研究发展计划(863)：肿瘤细胞泛素-蛋白酶体降解途径中相关新蛋白筛选技术研究。

11. 北京市自然科学基金委员会：胰腺癌生物标志物筛选。

12.   北京市自然科学基金委员会：几种常见肿瘤的分子标志谱建立及在预警、诊治和预后中的应用-胰腺癌分子标志谱的研究与分离鉴定肿瘤相关蛋白技术平台的建立。

1.  Mo Zhang, Qing Wang, Yupin Xu, Lei Guo, Zhizhen Lai and Zhili Li*, Graphitic carbon nitride quantum dots as analytical probe forviewing sialic acid on the surface of cells and tissues, Analytica Chimica Acta, 2020,1095,204-211.

2.  Dan Zhang, Xiaoou Li, Xiaofeng Liu, Yanmin Wang, Mo Zhang, QinWang, Tianjing Chen, Zhili Li*, Disease-specificIgG Fc glycosylation ratios as personalized biomarkers to differentiatenon-small cell lung Cancer from benign lung diseases, Proteomics Clinical Applications,2020,14,1900016.

3.  Na Li, Yuming Qiu, Yunpeng Wu, Mo Zhang, Zhizhen Lai, Qing Wang,Yuying Du, Lei Guo, Shuai Liu, Zhili Li*,Association of serum total fatty acids with type 2 diabetes, ClinicaChimica Acta, 2020, 500, 59-68.

4.  Yunpeng Wu, Rui Zhou, Zhizhen Lai1, Wei Wang, Na Li, YuyingDu, Lei Guo, Yuming Qiu, Qing-Tao Wang*, and Zhili Li*，Monitoring novel modified hemoglobinusing mass spectrometry contributes to accurate blood glucose management of theHan Chinese population, Clinica Chimica Acta, 2019, 489,124-129.

5. Tianjing Chen, Chengyan He, Mo Zhang, Xiaoou Li, Xiaofeng Liu,Yujie Liu, Dan Zhang, and Zhili Li*, Disease-specific haptoglobin-βchain N-glycosylation as biomarker to differentiate non-small celllung cancer from benign lung diseases, Journal of Cancer, 2019, 10,5628-5637.

6.  Y. H. Sun, J. Li, H. J. Shu, Z. L. Li, J. M. Qian, Serumimmunoinflammation‑related protein complexes discriminate between inflammatory boweldisease and colorectal cancer, Clinical and Translational Oncology,2019, 21, 1680-1686.

7.   Shuai Guo, Yanmin Wang, Dan Zhou, Yupin Xu, Tianjing Chen, Zhili Li*, Association of alteration of nucleosides and nucleotides withgastric cancer microenvironment, International Journal of Mass Spectrometry,2018, 434, 37–42.

8.  Yupin Xu, Mo Zhang, Qing Wang, Zhili Li*, In situdetecting changes in membrane lipid phenotypes of macrophages cultured indifferent cancer microenvironments using mass spectrometry, AnalyticaChimica Acta, 2018, 1026, 101-108. 

9. Yujie Liu, Yunpeng Wu, Yanmin Wang, Mo Zhang,and Zhili Li*，IncreasedLevels of Serum Protein Complexes Are Associated with Type 2 Diabetes，International Journal ofMedical Sciences, 2018, 15(3), 210-216.

10.  Mo Zhang, Yujie Liu, Dan Zhang,Tianjing Chen, Zhili Li*, Facileand Selective Enrichment of Intact Sialoglycopeptides Using Graphitic CarbonNitride, Analytical Chemistry, 2017, 89(15) , 8064-8069. 

11.  Shuai Guo, Dan Zhou, Mo Zhang, TiejunLi, Yujie Liu, Yupin Xu, Tianjing Chen, ZhiliLi*, Monitoring changes of docosahexaenoic acid-containing lipids duringthe recovery process of traumatic brain injury in rat using mass spectrometry imaging,ScientificReports, 2017, 7. e5054.

12.  Yumei Guo, Junling Ren, Xiaoou Li,Xiaofeng Liu, Ning Liu, Yanmin Wang, ZhiliLi*, Simultaneous Quantification of Serum Multi-Phospholipids as PotentialBiomarkers for Differentiating Different Pathophysiological states of lung,stomach, intestine, and pancreas, Journal of Cancer, 2017, 8(12),2191-2204. 

13. Dan Zhou, Shuai Guo, Mo Zhang, YujieLiu, Tianjing Chen, Zhili Li*,Mass spectrometry imaging of small molecules in biological tissues usinggraphene oxide as a matrix, Analytica Chimica Acta, 2017, 962,52-59.

14.  Xiaodong Wang, Yujie Liu, Fenjie Li, Zhili Li*, Poplarcatkin: a natural biomaterial for highly specific and efficient enrichment of sialoglycopeptides, ChineseChemical Letter, 2017, 28,1018-1026.

15. Chenchen Xu, Dan Zhou, Yixin Luo, ShuaiGuo, Tao Wang, Jie Liu*, Yuehua Liu*, ZhiliLi*, Tissue and serum lipidome shows altered lipidcomposition with diagnostic potential in mycosis fungoides, Oncotarget, 2017, 8,48041-48050.

16. JunlingRen, Dan Zhang, Yujie Liu, Ruiqing Zhang, Huiling Fang, Shuai Guo, Dan Zhou, MoZhang, Yupin Xu, Ling Qiu*, Zhili Li*,Simultaneous Quantification of Serum Nonesterified and Esterified Fatty Acidsas Potential Biomarkers to Differentiate Benign Lung Diseases from Lung Cancer,ScientificReports, 2016, 6, e34201. 

17. DanZhang, Bingchao Chen, Yanmin Wang, Peng Xia, Chengyan He, Yujie Liu, RuiqingZhang, Mo Zhang, Zhili Li* Disease-specificIgG Fc N-glycosylation as personalized biomarkers to differentiate gastriccancer from benign gastric diseases, Scientific Reports, 2016,6, e25957.

18. ManwenHe, Shuai Guo, Junling Ren, Zhili Li*,In Situ Characterizing Membrane Lipid Phenotype of Human Lung Cancer Cell LinesUsing Mass Spectrometry Profiling,Journalof Cancer, 2016, 7(7), 810-816.

19. YapingZhang, Chengyan He, Ling Qiu, Yanmin Wang, Xuzhen Qin, Yujie Liu, Zhili Li*, Serum unsaturated free fattyacids: a potential biomarker panel for early-stage detection of colorectalcancer, Journal of Cancer, 2016, 7(4), 477-483.

20. YujieLiu, Yuanjun Liu, Dan Zhang, Ruiqing Zhang, Zhili Li*, Kapok fiber:a natural biomaterial for highly specific and efficient enrichment of sialoglycopeptides,AnalyticalChemistry, 2016, 88(2),1067-1072.

 21.  Yujie Liu, Dan Zhang, Yuhang Cheng*, Zhili Li*, Elevated serum immunoinflammation-relatedprotein complexes are associated with psychosis, PsychiatryResearch, 2015, 230, 96-101.

22. YapingZhang, Ling Qiu, Chengyan He, Yanmin Wang, Yujie liu, Dan Zhang, Zhili Li*, Serum unsaturatedfree fatty acids: a potential biomarker panel for differentiating benignthyroid diseases from thyroid cancer, Journal of Cancer, 2015,6(12), 1276-1281.

23.  ShuaiGuo, Yanmin Wang, Dan Zhou, and Zhili Li*,Electric Field-Assisted Matrix Coating MethodEnhances the Detection of Small Molecule Metabolites for Mass SpectrometryImaging, AnalyticalChemistry, 2015, 87(12), 5860-5865. 

24. ManwenHe, Shuai Guo, Zhili Li*, In situcharacterizing membrane lipid phenotype of breast cancer cells using massspectrometry profiling, Scientific Reports, 2015, 5, e11298, 

25. ManwenHe, Shuai Guo, Zhili Li*, Dynamicallyin situ monitoring lipid changes in DHA-treated breast cells by matrix-assistedlaser desorption/ionization Fourier transform ion cyclotron resonance massspectrometry, Rapid Commun Mass Spectrom, 2015, 29(10), 987-990.

26. Gaoguang Song, Yujie Liu, Yanying Wang,Guanjun Ren, Shuai Guo, Junling Ren, Li Zhang*, ZhiliLi*, Personalized biomarkers to monitordisease progression in advanced non-small-cell lung cancer patients treated with icotinib, Clinica Chimica Acta, 2015,440, 44-48.

 27. YapingZhang, Ling Qiu, Yanmin Wang, Chengyan He, Xuzhen Qin,Yujie liu, Zhili Li*, Unsaturated free fatty acids: a potential biomarker panel forearly detection of gastric cancer, Biomarkers,2014, 19(8),667-673.

28. ShuaiGuo, Yanmin Wang, Dan Zhou, ZhiliLi*, Significantlyincreased monounsaturated lipids relative to polyunsaturated lipids in sixtypes of cancer microenvironment are observed by mass spectrometry imaging,ScientificReports, 2014, 4, e5959.

29.  YapingZhang, Chengyan He, Ling Qiu, Yanmin Wang, Li Zhang, Xuzhen Qin, Yujie Liu, DanZhang, Zhili Li*, Serum Unsaturated Free Fatty Acids: Potential Biomarkersfor Early Detection and Disease Progression Monitoring of Non-SmallCell Lung Cancer, Journal of Cancer,2014, 5(8), 706-714. 

30.  YapingZhang, Lina Song, Ning Liu, Chengyan He*,Zhili Li*, Decreasedserum levels of free fatty acids are associated with breast cancer, Clinica Chimica Acta, 2014, 437, 31-37. 

31. Shuai Guo, Ling Qiu, Yanming Wang, Xuzhen Qin, Hui Liu,Manwen He, Yaping Zhang, Zhili Li*and Xiaohong Chen*, Tissue imaging and serum lipidomic profiling for screeningpotential biomarkers of thyroid tumors by matrix-assisted laserdesorption/ionization-Fourier transform ion cyclotron resonance massspectrometry, Analyticaland Bioanalytical Chemistry, 2014, 406, 4357-4370.

32.  Yaping Zhang, Ling Qiu, Yanmin Wang, Xuzhen Qin, Zhili Li*, High-throughputand high-sensitivity quantitative analysis ofserum unsaturated fatty acids by chip-based nanoelectrosprayionization-Fourier transform ion cyclotron resonance mass spectrometry: early stage diagnosticbiomarkers of pancreatic cancer. Analyst, 2014, 139(7), 1697-1706.

33. Yanying Wang, Gaoguang Song, Yanmin Wang, Ling Qiu, XuzhenQin, Hui Liu, Fang Li, Xiaodong Wang, Fenjie Li, Shuai Guo, Yaping Zhang, Zhili Li*, Elevated serum levels of circulating immunoinflammation-relatedprotein complexes are associated with cancer. Journal of ProteomeResearch, 2014, 13 (2), 710–719.

34. Guoqiang Chen, Hexiang Li, Ling Qiu, Xuzhen Qin, Hui Liu, Zhili Li*, Change in fucosylated IgG2 Fc-glycoforms inpancreatitis and pancreatic adenocarcinoma: A promising disease classification model. Analyticaland Bioanalytical Chemistry, 2014, 406, 267-273. 

35. Yaping Zhang, Yanmin Wang, Shuai Guo, Yumei Guo, Hui Liu, Zhili Li*, Ammonia-treated N-(1-naphthyl) ethylenediaminedihydrochloride as a novel matrix for rapid quantitative and qualitativedetermination of serum free fatty acids by matrix-assisted laser desorption/ionization-Fouriertransform ion cyclotron resonance mass spectrometry, Analytica Chimica Acta, 2013, 974, 82-89. 

36. Fenjie Li,Xiaodong Wang, Yujie Liu, Hui Liu, ZhiliLi* Dephosphorylationof Intact Glycoprotein to Greatly Improve Digestion Efficiency Coupledwith Matrix-Assisted Laser Desorption/Ionization- Fourier Transform Ion CyclotronResonance Mass Spectrometric Analysis. Analytica Chimica Acta, 2013, 787, 140-147. 

37.Guoqiang Chen, YanminWang, Xuzhen Qin, Hexiang Li, Yumei Guo, Yanying Wang, Hui Liu, Xiaodong Wang,Gaoguang Song, Fang Li, Fenjie Li, Shuai Guo, Ling Qiu, Zhili Li* Change in IgG1 Fc N-linked glycosylation in human lung cancer: Age and sex-related diagnostic potential. Electrophoresis 2013, 34 (16),2407-2416.

38. Guoqiang Chen, Li Zhang,Yanying Wang, Yumei Guo, Hui Liu, ZhiliLi*. Probinggender-specific changein IgG1 Fc-galactosylation in non-small cell lung cancer patients treated withepidermal growth factor receptor inhibitor, icotinib hydrochloride, usingmatrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonancemass spectrometry, Rapid Commun Mass Spectrom 2013, 27(10), 1168-1172.

39.  FangLi, Xuzhen Qin, Haiquan Chen, Ling Qiu, Yumei Guo, Hui Liu, Guoqiang Chen GaoguangSong, Xiaodong Wang, Fenjie Li, Shuai Guo, Baohua Wang and Zhili Li*, Lipid profiling for early diagnosis and progression ofcolorectal cancer using direct-infusion electrospray ionization Fouriertransform ion cyclotron resonance mass spectrometry, Rapid Communications in Mass Spectrometry,2013, 27(1), 24-34.

40. YumeiGuo, Xianmin Wang, Ling Qiu, Xuzhen Qin, Hui Liu, Yanying Wang, Fang Li,Xiaodong Wang, Guoqiang Chen, Gaoguang Song, Fenjie Li, Shuai Guo, Zhili Li*, Probinggender-specific lipid metabolites and diagnostic biomarkers for lung cancerusing Fourier transform ion cyclotron resonance mass spectrometry, ClinicaChimica Acta, 2012, 414, 135-141.

41. XiaodongWang, Fenjie Li, Gaoguang Song, Shuai Guo, Hui Liu, Guoqiang Chen, Zhili Li*, Broad-spectrum four-dimensionalorthogonal electrophoresis: a novel comprehensively feasible system for proteincomplexomics investigation, Molecular & Cellularproteomics, 2012, 11(9), 786-799.

42. GuoqiangChen, Yanmin Wang, Ling Qiu, Xuzhen Qin, Hui Liu, Xiaodong Wang, Yanying Wang,Gaoguang Song, Fang Li, Yumei Guo, Fenjie Li, Shuai Guo, Zhili Li*, Human IgG Fc-glycosylation profilingreveals association with age, sex, female sex hormones and thyroid cancers, Journalof Proteomics, 2012, 75(10), 2824-2834.

43. LingXin, Haijing Zhang, Hui Liu, Zhili Li*, Equal ratio ofgraphite carbon to activated charcoal for enrichment of N-glycopeptides priorto matrix-assisted laser desorption/ionization time-of-flight massspectrometric identification, Rapid Communications in Mass Spectrometry,2012, 26(3), 269–274.

44. Wang,Xin, Zhang, Mei Fen, Xie, Jing, Li, Zhi Li, Wang, Peng*, Behcet'sDisease with Active Uveitis: Detection of Serum Protein Biomarkers UsingMALDI-TOF-MS, Anatomical Record, 2012, 295(7), 1168-1173.

1.   评估肿瘤药物治疗的个体化标志物及其应用；专利申请号：202110198218.2；发明人：李智立、李丹、张慧娟、张继匀。

2. . IgG Fc N-糖肽作为确定或辅助肺癌治疗方案选择的标志物的用途；专利申请号：202110193804.8；发明人：李智立、周巾煜、杜钰莹。

3. 一种富集糖肽的磁性聚苯胺纳米材料的制备方法和应用；专利申请号：202011355930；发明人：李智立、赖治臻、张沫。

4. 一种苯硼酸修饰氮化碳量子点的制备方法及其应用；专利申请号：201910433447；发明人：李智立、张沫。

5. 一种基于氧化铜/氮化碳无酶葡萄糖传感器的制备方法及应用；专利申请号：201910269990.1；发明人：李智立、张沫。

6. 一种富集分离唾液酸化糖肽的新材料及其应用；专利号：ZL201610506699.8；发明人：李智立、张沫。

7.  确定或辅助监测癌症进展的个体化标志物；专利号：ZL201610369521.3；发明人：李智立、张瑞青。

8.    一种富集唾液酸化糖肽的材料及其应用；专利号：ZL201510651116.6；发明人：李智立、刘育杰。

9.   一种原位质谱分析的常压离子源装置；专利号：ZL201520119228.2；发明人：李智立、郭帅。

10.    一种用于质谱成像的基质喷涂装置；专利号：ZL201420766737.x；发明人：李智立、郭帅。

11.    一种富集唾液酸化糖肽、唾液酸化聚糖或唾液酸化糖苷的方法； 专利号：ZL201410035250.9；发明人：李智立、王晓东。

12.   蛋白质复合物及其谱以及其在疾病诊断、进展评估和药效评估中的应用；申请号：PCT/CN2012/071113；发明人：李智立、王彦英。

13.   蛋白质复合物及其在肿瘤诊断和/或预后评估中的应用；专利号：ZL201110037632.1；发明人：李智立、王彦英。

14.   一种多发性骨髓瘤特异性蛋白及其专用检测试剂盒；专利号：ZL201010238794.7；发明人：李智立、张海靖、刘辉。

15.  一种糖肽富集分离材料及其应用；专利号：ZL2010101195689.x；发明人：李智立、辛玲。